Word on the street is that "gtk-enable-mnemonics" has been deprecated since version 3.10, and I am not at all sure how to get my application to display mnemonics. They will only display if I press the alt key. Please see here: http://stackoverflow.com/questions/23049406/wxpython-button-shortcut-accelerator-how-to-spam I have tried everything suggested in this article in regards to settings.ini, switching the bool to the opposite: How do I disable mnemonics in GTK3? Related: https://developer.gnome.org/gtk3/3.2/GtkSettings.html